酶聯(lián)免疫法測(cè)定，用于定量測(cè)定血漿和血清中的鈣視網(wǎng)膜蛋白。僅供科研使用。
Enzyme Immuno Assay for the Quantitative Determination of Calretinin in Plasma and Serum

臨床領(lǐng)域：腫瘤學(xué)、癌癥篩查、職業(yè)病
Clinical Fields：Oncology, Cancer screening, Occupational diseases

疾?。洪g皮瘤（中國(guó)是間皮瘤死亡人數(shù)最多的國(guó)家。據(jù)估計(jì)，2017年中國(guó)間皮瘤死亡人數(shù)超過(guò)1萬(wàn)人[1]）
Diseases：Mesothelioma（China is the leading country in terms of mesothelioma death. The estimated number of mesothelioma deaths in China in 2017 was more than 10,000[1]）

規(guī)格：微孔板（12 x 8），預(yù)包被多克隆鈣視網(wǎng)膜蛋白抗體
Format：Microtiter plate (12 x 8 determinations, single break apart wells), coated with polyclonal Calretinin antibodies

樣品量：15μl患者血清或血漿
Sample volume: 15 μl patient serum or plasma

樣品制備：室溫下60分鐘振蕩
Sample preparation: 60 min shaking at room temperature
ELISA：室溫下4.5小時(shí)培養(yǎng)，450nm下ELISA讀板機(jī)的測(cè)量值（參考值620nm）
ELISA: 4.5 hours incubation at room temperature
Measurement in ELISA reader at 450 nm (620 nm reference)
6個(gè)標(biāo)準(zhǔn)品，范圍：0.25 ng/ml - 4.0ng/ml
6 standards with range: 0.25 ng/ml - 4.0 ng/ml
2個(gè)試劑盒質(zhì)控品，凍干
2 kit controls, lyophilized
酶標(biāo)記抗體濃縮200x，洗滌液濃縮50x
Enzyme conjugate 200x concentrated, wash buffer 50x concentrated
所有其他試劑可立即使用
All other reagents ready for use

特性：靈敏度59%，特異性95%
Specificity：95 % Specificity at 59 % Sensitivity

特性：適用于大批量血清或血漿樣品測(cè)試，經(jīng)過(guò)大型病例對(duì)照研究驗(yàn)證，參與前瞻性研究

Characteristics：Suitable for testing large series of serum or plasma samples
Validated in larger case-control studies
Involved in prospective studies

參考文獻(xiàn)：[1]Furuya S, Chimed-Ochir O, Takahashi K, et al. Global Asbestos Disaster[J]. International Journal of Environmental Research & Public Health, 2018, 15(5):1000-.